Peptide Cheat Sheet: Your Complete Dosing & Reconstitution Guide
This guide is provided for educational purposes only and offers a general overview of peptide reconstitution and dosing calculations. This information does not replace advice from your GP or healthcare provider. Always consult a qualified medical professional before starting, adjusting, or discontinuing any peptide protocol.
What Are Peptides?
Peptides are short chains of amino acids that act as biological messengers in the body. Depending on the peptide, they can support fat loss, longevity, healing, immunity, cognitive function, sexual health, muscle building, and sleep. At The Skinny, we've put together this comprehensive cheat sheet to help you understand how to reconstitute and dose the most commonly used peptides.
Step 1: How to Reconstitute a Peptide
Reconstitution is the process of mixing a freeze-dried (lyophilised) peptide powder with bacteriostatic water (BAC water) to create an injectable solution. Follow these steps carefully:
- Clean Surfaces: Sanitize your workspace and wash your hands thoroughly before handling any vials or syringes.
- Disinfect Vial Tops: Use a fresh alcohol swab on both the peptide vial and the bacteriostatic water vial before every use.
- Draw Bacteriostatic Water: Using a sterile black 3ml syringe, withdraw the required amount of BAC water as per the dosing table below.
- Inject into the Peptide Vial: Insert the needle at an angle and inject the BAC water slowly down the side of the vial to prevent foaming.
- Swirl Gently: Gently swirl the vial until the powder is fully dissolved. Never shake the vial — this can degrade the peptide.
Peptide Dosing & Reconstitution Table
Use the table below as a reference guide. All dosing examples are based on commonly used protocols and are for educational purposes only.
🔴 Fat Loss Peptides
| Peptide | Amount in Vial | BAC Water | Recommended Dosage | Units on 1ml Syringe | Timing | Frequency | Duration |
|---|---|---|---|---|---|---|---|
| Ipamorelin | 5mg | 2ml | 250mcg | 10 | AM/PM | 5 days on, 2 days off | 8 weeks on, 8 weeks off |
| Tesamorelin | 5mg | 2ml | 1000mcg | 40 | AM/PM | 5 days on, 2 days off | 8 weeks on, 8 weeks off |
| AOD-9604 | 2mg | 2ml | 300mcg | 30 | AM | 5 days on, 2 days off | 8 weeks on, 8 weeks off |
| Semaglutide | 6mg | 2ml | 250mcg | 12.5 | AM | Once / Week | 8 weeks on, 8 weeks off |
| Tirzepatide | 30mg | 3ml | 2500mcg | 25 | AM | Once / Week | 8 weeks on, 8 weeks off |
| Retatrutide | 32mg | 3ml | 2000mcg | 19 | AM | Once / Week | 8 weeks on, 8 weeks off |
| MOTS-C | 5mg | 2ml | 2000mcg | 80 | AM | Every 3rd Day | 2 Weeks |
| Ipamorelin / CJC-1925 No DAC | 12mg | 3ml | 300mcg | 7.5 | AM/PM | 5 days on, 2 days off | 8 weeks on, 8 weeks off |
🟢 Longevity Peptides
| Peptide | Amount in Vial | BAC Water | Recommended Dosage | Units on 1ml Syringe | Timing | Frequency | Duration |
|---|---|---|---|---|---|---|---|
| CJC-1925 No DAC | 10mg | 3ml | 200mcg | 6 | PM | 5 days on, 2 days off | 8 weeks on, 8 weeks off |
| CJC 1925 DAC | 2mg | 2ml | 250mcg | 20 | PM | Every 3rd Day | 8 weeks on, 8 weeks off |
| Epitalon | 100mg | 3ml | 1000mcg | 30 | AM | 3 Days / Week | 3 weeks straight, once per year |
| Thyamlin | 100mg | 3ml | 5000mcg | 15 | AM | 20 Days Straight | Once every 6 months |
| SS-31 | 10mg | 2ml | 500mcg | 10 | AM | 5 days on, 5 days off | 8 weeks on, 8 weeks off |
| NAD+ | 500mg | 2ml | 100mg | 40 | AM | 2-3 Days per Week | As Needed |
🟡 Healing Peptides
| Peptide | Amount in Vial | BAC Water | Recommended Dosage | Units on 1ml Syringe | Timing | Frequency | Duration |
|---|---|---|---|---|---|---|---|
| GHK-CU | 5mg | 2ml | 1500mcg | 5 | AM | Every Day | 8 weeks on, 8 weeks off |
| BPC-157 | 5mg | 2ml | 250mcg | 7.5 | AM/PM | Every Day | 8 weeks on, 8 weeks off |
| TB-500 | 5mg | 2ml | 2000mcg | 60 | AM | Every Other Day | 8 weeks on, 8 weeks off |
🔵 Immunity Peptides
| Peptide | Amount in Vial | BAC Water | Recommended Dosage | Units on 1ml Syringe | Timing | Frequency | Duration |
|---|---|---|---|---|---|---|---|
| Thymosin Alpha 1 | 10mg | 3ml | 1500mcg | 45 | AM | 2 Days per Week | 8 weeks on, 8 weeks off |
| LL-37 | 5mg | 2ml | 125mcg | 5 | AM | Every Day | 50 days straight, 4 weeks off |
| VIP | 5mg | 5ml | 50mcg | 5 | AM/PM | Every Day | 8 weeks on, 8 weeks off |
| KPV | 10mg | 3ml | 250mcg | 7.5 | AM | 5 days on, 2 days off | 8 weeks on, 8 weeks off |
🟣 Cognition & Brain Health Peptides
| Peptide | Amount in Vial | BAC Water | Recommended Dosage | Units on 1ml Syringe | Timing | Frequency | Duration |
|---|---|---|---|---|---|---|---|
| Melanotan 1 | 10mg | 3ml | 250mcg | 7.5 | AM | 2 Days per Week | 8 weeks on, 8 weeks off |
| Semax or NA Semax Amidate | 30mg | 3ml | 1000mcg | 10 | AM | 2-3 Days per Week | 8 weeks on, 8 weeks off |
| Selank or NA Selank Amidate | 30mg | 3ml | 1000mcg | 10 | AM | 2-3 Days per Week | 8 weeks on, 8 weeks off |
🩷 Sexual Health Peptides
| Peptide | Amount in Vial | BAC Water | Recommended Dosage | Units on 1ml Syringe | Timing | Frequency | Duration |
|---|---|---|---|---|---|---|---|
| PT-141 | 10mg | 2ml | 500mcg | 10 | 30 min before sex | As Needed | As Needed |
| Oxytocin | 10mg | 10ml | 50mcg | 5 | AM | As Needed | As Needed |
| Kisspeptin | 5mg | 3ml | 100mcg | 6 | 1 Hour Before Bed | Every Day | 30 days on, 30 days off |
💪 Muscle Building
| Peptide | Amount in Vial | BAC Water | Recommended Dosage | Units on 1ml Syringe | Timing | Frequency | Duration |
|---|---|---|---|---|---|---|---|
| IGF-ILR3 | 1mg | 1ml | 50mcg | 5 | AM | 10 Days in a Row | 10 days on, 4 weeks off |
😴 Sleep
| Peptide | Amount in Vial | BAC Water | Recommended Dosage | Units on 1ml Syringe | Timing | Frequency | Duration |
|---|---|---|---|---|---|---|---|
| DISP | 5mg | 3ml | 100mcg | 6 | 1-3 Hours Before Bed | 5 days on, 2 days off | 8 weeks on, 8 weeks off |
1ml Syringe Note: Each line on a 1ml syringe represents 0.01ml, making it the most precise syringe for measuring small doses of medication.
Step 2: How to Prepare & Administer a Subcutaneous Injection
Prepare for Your Injection
- Wash your hands thoroughly with soap and water.
- Assemble your supplies: a needle, a medication vial, and an alcohol pad.
- Choose an injection site — upper arm, abdomen, or thigh.
NB: Rotate your injection site to keep the skin healthy. Avoid the navel, waistline, scars, and moles.
Draw Up & Administer the Injection
- Clean the exposed top of the vial with an alcohol swab before every use.
- Remove the plastic plunger cover at the bottom of the syringe.
- Remove the plastic needle cover to expose the needle.
- Pull back the plunger to fill the syringe with air equal to the amount of medication you'll be drawing.
- With the vial upright, push the needle straight down through the centre of the vial top. DO NOT BEND THE NEEDLE. If the needle bends, dispose of the entire syringe and start again.
- Push the air into the vial, then slowly pull back the plunger to draw your correct dose. If you see air bubbles, push the medication back into the vial and draw again slowly.
- Clean the injection site with a fresh alcohol pad.
- Pinch the skin at the injection site and inject at a 90-degree angle.
- Dispose of the needle safely and correctly after use.
Final Reminder
This cheat sheet is a starting point — not a prescription. Peptide protocols should always be personalised to your health goals, body weight, and medical history. Always work with a qualified healthcare provider before beginning any peptide protocol.
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